Debra Irwin, 56, and her grand-daughter, three-year-old Fallon Painter, traveled west on County Road 455 at around 3:45 p.m. when the crash occurred.
James Eric Walker, 22, lost control of his Jaguar car and crashed into the oncoming SUV driven by Irwin near State Road 19.
Irwin and Walker died at the scene of the crash, according to WESH. The three-year-old girl received treatment at Arnold Palmer Hospital but succumbed to her injuries.
Police say Walker had a suspended license dating back to last July, reports WESH.
The following information is provided by the National Highway Traffic Safety Administration (NHTSA).